Isikhathi se-Thrombin (TT) kanye nesikhathi se-prothrombin (PT) zivame ukusetshenziswa njengezinkomba zokuthola umsebenzi we-coagulation, umehluko phakathi kwalokhu okubili usekutholakaleni kwezinto ezahlukene ze-coagulation.

Isikhathi se-Thrombin (TT) siyinkomba yesikhathi esidingekayo ukuthola ukuguqulwa kwe-plasma prothrombin ibe yi-thrombin. Sisetshenziswa kakhulu ukuhlola isimo somsebenzi we-fibrinogen kanye nezinto ezihlanganisa i-coagulation I, II, V, VIII, X kanye ne-XIII ku-plasma. Ngesikhathi senqubo yokuthola, inani elithile lama-prothrombin kanye nama-ion e-calcium ayangezwa ukuguqula i-prothrombin ku-plasma ibe yi-thrombin, bese kulinganiswa isikhathi sokuguqulwa, okuyinani le-TT.

Isikhathi se-Prothrombin (PT) siyinkomba yokuthola umsebenzi wezinto ezihlanganisa igazi ngaphandle kohlelo lokujiya kwegazi. Ngesikhathi senqubo yokuthola, inani elithile lokwakheka kwe-coagulation factor (njengezinto ezihlanganisa igazi II, V, VII, X kanye ne-fibrinogen) liyangezwa ukuze kusebenze uhlelo lokujiya kwegazi, bese kulinganiswa isikhathi sokwakheka kwe-clot, okuyinani le-PT. Inani le-PT libonisa isimo somsebenzi we-coagulation factor ngaphandle kohlelo lokujiya kwegazi.

Kufanele kuqashelwe ukuthi kokubili amanani e-TT kanye ne-PT yizinkomba ezivame ukusetshenziswa ukukala umsebenzi wokuqina kwegazi, kodwa lokhu kokubili akukwazi ukushintshana, futhi izinkomba zokuthola ezifanele kufanele zikhethwe ngokuya ngesimo esithile. Ngesikhathi esifanayo, kufanele kuqashelwe ukuthi umehluko ezindleleni zokuthola kanye nama-reagent ungathinta ukunemba kwemiphumela, futhi kufanele kuqashelwe ukusebenza okujwayelekile emtholampilo.